Plasmid

Part:BBa_K5048025:Design

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-14)


plasmid PBAD_QEP_GST


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1216
    Illegal XbaI site found at 323
    Illegal PstI site found at 1196
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1216
    Illegal PstI site found at 1196
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1216
    Illegal BglII site found at 1192
    Illegal BamHI site found at 239
    Illegal BamHI site found at 1182
    Illegal XhoI site found at 1188
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1216
    Illegal XbaI site found at 323
    Illegal PstI site found at 1196
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1216
    Illegal XbaI site found at 323
    Illegal PstI site found at 1196
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1395
    Illegal BsaI site found at 2462
    Illegal SapI site found at 56
    Illegal SapI.rc site found at 472
    Illegal SapI.rc site found at 3544


Design Notes

We add enterokinase site between Lpp'OmpA and small peptides in order to verify the enzymatic cleavage. We choose araBAD promoter instead of T7 promoter for better induction.


Source

The insertion fragment is complete synthesis . The backbone is purchased from Genscript.


References